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KMID : 0380020090240010075
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Korean Journal of Biotechnology and Bioengineering
2009 Volume.24 No. 1 p.75 ~ p.79
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Influence of Organic, Inorganic Nitrogen Sources and Amino Acids on the Biosynthesis of Coenzyme by Agrobacterium tumefaciens Mutant
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Kim Jeong-Keun
Won Yong-Bae
Lee Kang-Moon
Koo Yoon-Mo
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Abstract
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The effect of inorganic, organic nitrogen sources and amino acids on the coenzyme production and coenzyme component ratio was investigated. Among the nine organic nitrogen sources, CSP showed a remarkable enhancing effect on the production of coenzyme . But this enhancement was not observed in medium containing Bacto peptone, tryptone, casamino acid and soybean meal. These differences on the production of coenzyme may be due to differences in kinds and amounts of component amino acids and peptides in the various organic nitrogen sources tested. In the addition of inorganic nitrogens, only increase the coenzyme production by 2.0 times compare to the control group. The addition of L-tyrosine to the medium containing Bacto tryptone, was also determined to be crucial for the coenzyme production. But phenylalanin and tryptophan, other aromatic amino acids, had no stimulatory effect on the coenzyme production. These results show that the production and components ratio of coenzyme was greatly affected by the kinds and the concentration of inorganic, organic nitrogen sources as well as amino acids.
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KEYWORD
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Agrobacterium tumefaciens mutant, coenzyme , amino acids, tyrosine
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